Datasets & Resources from the Slavov Lab

We are committed to openly share our methodology and datasets, including metadata and computational pipelines needed for reproducing our research workflows. We also organized the formulation of community guidelines for performing, analyzing and reporting single-cell proteomics by mass spectrometry. Below are links to relevant resources from the Slavov Laboratory, the Single-cell Proteomics Center and collaborators.  

• Explore Interactively and Download Data
• Research Resources
• Protocols
• Research News

Explore Interactively and Download Data

• 2022 | pSCoPE: Prioritized single-cell proteomics
• 2021 | plexDIA: Increasing the throughput of sensitive proteomics
• 2021 | Droplet sample preparation for single-cell proteomics
• 2021 | Multiplexed single-cell proteomics using SCoPE2
• 2020 | Optimizing experiments using isobaric carriers
• 2019 | SCoPE2: Quantifying proteins in single cells with multiplexed mass-spec
• 2019 | DO-MS: Data-Driven Optimization of Mass Spectrometry Methods
• 2018 | Increasing single-cell proteome coverage by DART-ID
• 2018 | Automated sample preparation for high-throughput single-cell proteomics
• 2016 | Single-Cell ProtEomics by Mass Spectrometry (SCoPE-MS)
• 2016 | Post-transcriptional regulation across human tissues
• 2015 | Protein Composition of Ribosomes across Growth Conditions
• 2014 | Quantifying homologous proteins and proteoforms
• 2014 | Metabolic Remodeling during Exponential Cell Growth
• 2013 | Growth Deregulation and Aerobic Glycolysis
• 2011 | Metabolic Cycling without Cell Division Cycling
• 2010 | Transcriptional and metabolic growth rate response

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Resources

• Single-Cell Proteomics Methods, Data and Protocols
• GitHub repositories for code
• Single Cell Proteomics Center
• Technology dissemination
• Lab Protocols @ protocols.io
  

 

Protocols

• Multiplexed Single-Cell Proteomics by SCoPE2
  
• Sample preparation for single-cell proteomics
  
• Glucose-Limited Medium
  
• Ethanol-Limited Medium
  
• Phosphate-Limited Medium with Ethanol as a Sole Source of Carbon and Energy
  
• Nitrogen-Limited Medium with Ethanol as a Sole Source of Carbon and Energy
  
• Media Characterization
  
• Measuring heat and oxidative stress resistance of yeast cells
  
• Methanol-Chloroform Purification of Proteins
  
• Culturing Mouse Embryonic Stem Cells
  

News and Recently Published Research

News from the Single Cell Proteomics Center
Blog from Harvard Scholar
Ribosome Related Research Articles from bioRxiv, arXiv, and PubMed