Slavov Lab | Welcome new members

Goals

Help each lab member to grow as a scientist / engineer and become more independent
Learn to prioritize, to focus on the essential and ignore distractions
Learn to formulate and test hypotheses
Develop new skills
Make discoveries and develop methods that are conceptually original, distinctive & non-redundant.
Publish papers, advance our careers and raise support for the goals above.

Organization

Scheduling
- All scheduling is done via the lab google calendar that will be shared with you.
Data Sharing & Communication
- Announcement to the entire group are sent via the group email: quantlab@listserv.neu.edu
- We also communicate via lab slack account: https://slavovlab.slack.com/
- We share data and collaborate on manuscripts via google drive and google docs
- The slides for all lab presentations have to be placed in the designated google drive folder.
- We have a business lab google account with unlimited data storage for mass-spec data. It is research@slavovlab.net

Meetings

Weekly group meeting
- Summarize results from the last 1-2 months or lead a journal club discussion. The goal is to present your results more holisticaly in perspective. We rotate alphabetically according the order from our website.
- Get feedback from everybody and practice your presention skills.
Weekly club meetings
- Design experiments, set goals, get help with data analysis and interpretation
- Present less mature results to colleagues who need little background
- Update on goals set the previous week / month.
- To stay focused and organized, we follow Focus 20/20:
  - Start with the central point / goal of the presented work
  - Presenations are up to 20 min max.
General Meeting Time (GMT)
- Questions of limited interest for the rest of the group
- Detailed feedback on manuscripts, career development, et al.
- If you want a 1-1 meeting, book a slot within the GMT slots.

The meetings do not have to take the full allotted time. We generally book longer periods than I expect we will need.

Introductory resources

Mass-spectrometry

Ribosome Specialization

Videos
- Differential stoichiometry among core ribosomal proteins
Review Papers


	Slavov Laboratory \| Quantitative Biology Seeking principles in the coordination among protein synthesis, metabolism, cell growth and differentiation
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Welcome
Goals Help each lab member to grow as a scientist / engineer and become more independent Learn to prioritize, to focus on the essential and ignore distractions Learn to formulate and test hypotheses Develop new skills Make discoveries and develop methods that are conceptually original, distinctive & non-redundant. Publish papers, advance our careers and raise support for the goals above. Organization Scheduling All scheduling is done via the lab google calendar that will be shared with you. Data Sharing & Communication Announcement to the entire group are sent via the group email: quantlab@listserv.neu.edu We also communicate via lab slack account: https://slavovlab.slack.com/ We share data and collaborate on manuscripts via google drive and google docs The slides for all lab presentations have to be placed in the designated google drive folder. We have a business lab google account with unlimited data storage for mass-spec data. It is research@slavovlab.net Meetings Weekly group meeting Summarize results from the last 1-2 months or lead a journal club discussion. The goal is to present your results more holisticaly in perspective. We rotate alphabetically according the order from our website. Get feedback from everybody and practice your presention skills. Weekly club meetings Design experiments, set goals, get help with data analysis and interpretation Present less mature results to colleagues who need little background Update on goals set the previous week / month. To stay focused and organized, we follow Focus 20/20: Start with the central point / goal of the presented work Presenations are up to 20 min max. General Meeting Time (GMT) Questions of limited interest for the rest of the group Detailed feedback on manuscripts, career development, et al. If you want a 1-1 meeting, book a slot within the GMT slots. The meetings do not have to take the full allotted time. We generally book longer periods than I expect we will need. Introductory resources Mass-spectrometry Videos Introduction to mass-spectrometry experiments and their optimization by Gray Huffman Introduction to mass-spectrometry by Harrison Specht Introduction to mass-spectrometry by Nikolai Slavov Primer on single-cell proteomics by mass-spectrometry by Nikolai Slavov Review Articles Computational methods for understanding mass-spec data (Sinitcyn, Cox et al., 2018) Protein Analysis by mass-spec [small book] (Yates et al., 2013) Single-cell protein analysis by mass-spectrometry Perspective Articles Framework for multiplicative scaling of single-cell proteomics, Nature Biotechnology Research Briefing Advancing single-cell proteomics, JPR Perspective Single-cell proteomics, Science Perspective Ribosome Specialization Videos Differential stoichiometry among core ribosomal proteins Review Papers Ribosome Stoichiometry: From Form to Function (Emmott Jovanovic & Slavov, 2018)

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